mouse monoclonal anti human cxcr4 primary antibody  (R&D Systems)

Journal: Cell Division

Article Title: Ultrasonic microbubbles promote mesenchymal stem cell homing to the fibrotic liver via upregulation of CXCR4 expression

doi: 10.1186/s13008-023-00104-8

Figure Lengend Snippet: Cell surface expression of CXCR4 using FCM (n = 3). a – e Representative examples of the membrane expression levels of CXCR4 on MSCs in the different groups. The number of CXCR4-positive cells in the M + UTMB60s group ( c ) was significantly higher compared with the M group(a) and the M + U60s group ( b ). There was no significant difference in the number of CXCR4-positive cells between the M + UTMB60s group ( c ), the M + UTMB90s group ( d ) and the M + UTMB180s group ( e ). f Quantification of CXCR4 expression by FCM assay in the experimental MSCs. All values are expressed as the mean ± SD. **P < 0.01; n = 3

Article Snippet: The membrane was blocked with skim milk dissolved in TBST at room temperature for 2 h and then incubated overnight at room temperature with rabbit anti-CXCR4 primary antibody at a 1:500 dilution (Abcam, UK) and finally incubated with goat anti-rabbit HRP-conjugated secondary antibody at a 1:1000 dilution (Beyotime, China) for 1 h the next day.

Techniques: Expressing, Membrane

Journal: Cell Division

Article Title: Ultrasonic microbubbles promote mesenchymal stem cell homing to the fibrotic liver via upregulation of CXCR4 expression

doi: 10.1186/s13008-023-00104-8

Figure Lengend Snippet: Expression of CXCR4 by IHC (n = 10). The surface of CXCR4 immunohistochemistry positive cells is brown (× 200, scale bar = 25 μm). a – e IHC of CXCR4 in the M group, M + U60s group, M + UTMD 60 s group, M + UTMD 90 s group, M + UTMD 180 s group. The number of CXCR4-positive cells was relatively smaller in the M group ( a ) and the M + U60s group ( b ), P > 0.05. The percentage of cells expressing surface CXCR4 in the M + UTMB60s group ( c ) higher than the M + U60s group ( b ) and the M group ( a ), P < 0.05. There was no significant difference in the number of CXCR4-positive cells between the M + UTMB60s group ( c ), the M + UTMB90s group ( d ) and the M + UTMB180s group ( e ), P > 0.05. f Quantification of CXCR4 expression was performed by Image-Pro Plus 6.0 software. All values are expressed as the mean ± SD. **P < 0.01; n = 10

Article Snippet: The membrane was blocked with skim milk dissolved in TBST at room temperature for 2 h and then incubated overnight at room temperature with rabbit anti-CXCR4 primary antibody at a 1:500 dilution (Abcam, UK) and finally incubated with goat anti-rabbit HRP-conjugated secondary antibody at a 1:1000 dilution (Beyotime, China) for 1 h the next day.

Techniques: Expressing, Immunohistochemistry, Software

Journal: Cell Division

Article Title: Ultrasonic microbubbles promote mesenchymal stem cell homing to the fibrotic liver via upregulation of CXCR4 expression

doi: 10.1186/s13008-023-00104-8

Figure Lengend Snippet: Expression of CXCR4 by WB (n = 6). a WB of CXCR4 in the M group, M + U60s group, M + UTMD 60 s group, M + UTMD 90 s group, M + UTMD 180 s group. b Quantification of CXCR4 expression was performed by Image J software. All values are expressed as the mean ± SD. **P < 0.01; n = 6

Article Snippet: The membrane was blocked with skim milk dissolved in TBST at room temperature for 2 h and then incubated overnight at room temperature with rabbit anti-CXCR4 primary antibody at a 1:500 dilution (Abcam, UK) and finally incubated with goat anti-rabbit HRP-conjugated secondary antibody at a 1:1000 dilution (Beyotime, China) for 1 h the next day.

Techniques: Expressing, Software

Journal: Biochemical pharmacology

Article Title: The development of potent, competitive CXCR4 antagonists for the prevention of cancer metastasis.

doi: 10.1016/j.bcp.2023.115921

Figure Lengend Snippet: Fig. 6. IS4 binding to CXCR4 is similar to the binding of the CXCL12 mimetic compounds AMD3100 and peptide 10 to CXCR4. Immunofluorescence micro scopy of MCF-7 breast cancer cells shows positive expression of CXCR4 (green), visualized using either 12G5 mouse anti-CXCR4 monoclonal antibody (mAb) (binds to the extracellular loops 1 and 2 of CXCR4) or 4G10 mouse anti-CXCR4 mAb (binds to the N-terminus of CXCR4), plus secondary anti-mouse Alexa Fluor® 488 with nuclei indicated by DAPI staining (blue). No antagonist was used for positive controls. Negative control visualized using secondary anti- mouse Alexa Fluor® 488 and DAPI staining only. The CXCL12 mimetic com pounds AMD3100 and peptide 10 bind to similar residues on CXCR4 as compared to 12G5 therefore, in the presence of these compounds, fluorescence is lost. Fluorescence is restored with use of the 4G10 anti-CXCR4 mAb in place of 12G5. Data shows representative images from N = 4 acquired with Leica imaging suite using a 63x objective.

Article Snippet: Cells were washed twice in ice-cold 1X PBS then incubated with 1:200 primary mouse 12G5 anti-CXCR4 (sc-12764, Santa Cruz Biotechnology, Heidelberg, Germany) for 1 h at 4 ◦C.For negative control, no primary antibody was added.

Techniques: Binding Assay, Immunofluorescence, Expressing, Staining, Negative Control, Fluorescence, Imaging